Eddie Jones has named a 30-man training squad ahead of England's Autumn International fixture against Japan this weekend.
England have been stripped of second row George Kruis, who has been ruled the remainder of the international period with a calf injury. The 28-year-old started against South Africa and New Zealand earlier this month.
Suspended Saracens utility back Alex Lozowski is included and available for selection for the first time this season, having trained with the squad whilst banned from featuring against either the Springboks or the All Blacks after a dangerous tackle on Glasgow Warriors fullback Ruaridh Jackson in the Champions Cup.
There is no room in the squad for England's most capped fullback in history, Mike Brown, who misses out on selection for the first time this autumn.
Charlie Ewels (Bath Rugby)
Jamie George (Saracens)
Dylan Hartley (Northampton Saints)
Alec Hepburn (Exeter Chiefs)
Ted Hill (Worcester Warriors) *
Maro Itoje (Saracens)
Courtney Lawes (Northampton Saints)
Zach Mercer (Bath Rugby)
Ben Moon (Exeter Chiefs)
Nick Schonert (Worcester Warriors)
Brad Shields (Wasps)
Kyle Sinckler (Harlequins)
Elliott Stooke (Bath Rugby)
Sam Underhill (Bath Rugby)
Harry Williams (Exeter Chiefs)
Mark Wilson (Newcastle Falcons)
Chris Ashton (Sale Sharks)
Joe Cokanasiga (Bath Rugby)
Danny Care (Harlequins)
Elliot Daly (Wasps)
Owen Farrell (Saracens)
George Ford (Leicester Tigers)
Alex Lozowski (Saracens)
Jonny May (Leicester Tigers)
Jack Nowell (Exeter Chiefs)
Henry Slade (Exeter Chiefs)
Ben Te’o (Worcester Warriors)
Manu Tuilagi (Leicester Tigers)
Richard Wigglesworth (Saracens)
Ben Youngs (Leicester Tigers)
England will host Japan at Twickenham for the first time in history on Saturday (KO 3pm live on Sky Sports HD), facing the Wallabies the in the final fixture of the November period the following weekend.